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De Soto was the second expedition to penetrate into Florida's interior

Source: Early History of the Creek Indians and Their Neighbors #121

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The expedition of De Soto reached Tampa Bay May 25, 1539. On Tuesday, July 15, it set out from the town of Ocita, or Ucita, which was evidently near the head of the bay, passed through the territory of Mococo, and then through a number of places which seem to have been under a chief named Urriparacogi. Afterwards the explorers crossed the Withlacoochee River and came into the province of Ocale, and from there, leaving the province of Acuera to one side, reached the important province of Potano on or near the Alachua plains. Then they passed northward through Potano, crossed another river, perhaps the Santa Fe, and came into still another important province known as Aguacalecuen, or Caliquen. It is uncertain whether the places entered by them, beyond the capital of this province, all belonged to it or not. At any rate the next great chief mentioned was Ucachile, Uzachil, or Ossachile, a name which I have sought to identify with the later Osochi, and from his territory they traveled into the province of Apalachee northward of Ocilla River.4 All of the people living in these places probably belonged to the great Timucua group. The De Soto chroniclers are of particular service in giving us an early picture of the tribes of this stock toward the western side of the peninsula, the later settlements all having been made from the east.
(Swanton)