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The Cusabo had a population of 535 Indians mixed with the English settlements

Source: Early History of the Creek Indians and Their Neighbors #121

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POPULATION OF THE SOUTHEASTERN TRIBES
The population of an Indian tribe at any early period in its history can not be determined with exactness. In the case of the Creeks we have to consider not only the Muskogee or Creeks proper, but a number of tribes afterwards permanently or temporarily incorporated with them, and the problem is proportionately complicated. Fortunately we are helped out by a considerable number of censuses, some of which were taken with more than usual care.

The Cusabo tribes were always small, even at the time of their first intercourse with the Spaniards and French, but we have no data regarding their population until the year 1715, just before the outbreak of the Yamasee war, when a careful estimate approaching an actual enumeration as closely as was possible at that time was made under the auspices of Governor Johnson of South Carolina. There were then two bands left belonging to this group. The "Corsaboys" (i.e., the Cusabo proper) are credited with five villages, 95 men, and a total population of 295, while the Itwans of Charleston Entrance had but one village, with 80 men, and a total population of 240.3 The entire population of this group was therefore 535, and they are already described as "mixed with the English settlement." The Yamasee war depleted their numbers considerably. Most of them probably remained in the same place, where they progressively declined and disappeared, though a few retired among the inland Indians. The Coosa are not separately enumerated in this list, and it is uncertain whether they were omitted or are included among the Cusabo. According to Adair some of them later joined the Catawba, but probably not all.4
(Swanton)